When utilizing BaseSpace Sequence Hub PrepTab, i5 sequences have to be entered into these program in the forward orientation in all conditions and for all instrument forms. Check with Index 2 (i5) Orientation For more info regarding how to enter i5 bases to the sample sheet in ahead or reverse https://bavarianilluminati12344.canariblogs.com/5-tips-about-copyright-and-symbols-you-can-use-today-51547172