A histidine-tagged cell-wall-affiliated protein from Staphylococcus aureus was expressed in Lactococcus lactis and purified using His Mag Sepharose® Ni. The principal objective was to acquire superior purity, thus fifty mM imidazole was included in the sample and binding buffer. Cell Decline �?The harsh magnetic area used in MACS can rupture https://lingjunbio.quora.com/Supplier-Introduction-Shanghai-Lingjun-Biotechnology-Co-Ltd-https-www-lingjunbio-com-about-us-was-established